shRNA library

Details shRNA library shRNA library

2011-01-04

2011-01-04

Lundgren, Jeffrey S (Department: 1639)

Combinatorial chemistry technology: method, library, apparatus

Method of creating a library

Biochemical method

C435S091200, C506S017000, C506S010000

Reexamination Certificate

active

07863222

ABSTRACT:
As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2wherein, in each nucleic acid of the plurality: S1and S2are each at least 15 nucleotides in length; S1and S2are complementary to each other along their entire length; either S1or S2is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1and S2vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

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