Sequence-directed DNA-binding molecules compositions and methods

Details Sequence-directed DNA-binding molecules compositions and methods Sequence-directed DNA-binding molecules compositions and methods

1995-06-07

1998-04-28

Zitomer, Stephanie W.

Drug, bio-affecting and body treating compositions

Solid synthetic organic polymer as designated organic active...

436501, 514 1, A61K 3174, G01N 33566, G01N 33558

Patent

active

057441316

ABSTRACT:
The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

REFERENCES:
patent: 4257774 (1981-03-01), Richardson et al.
patent: 4270924 (1981-06-01), Crooke et al.
patent: 4665184 (1987-05-01), Dervan et al.
patent: 4942227 (1990-07-01), Dervan et al.
patent: 5071773 (1991-12-01), Evans et al.
patent: 5096815 (1992-03-01), Ladner et al.
Davison, A.J., and Scott, J.E., "The Complete DNA Sequence of Varicella-Zoster Virus," J. Gen. Virol. 67:1759-1816 (1986).
Hanvey, J.C., et al., "Site-specific inhibition of EcoRl restriction/modification enzymes by a DNA triple helix," Nucleic Acids Res. 18(1):157-161 (1990).
Hobson, K., et al., "Use of DNA-Protein Interaction to Isolate Specific Genomic DNA Sequences," Anal. Biochem. 193:220-224 (1991).
McGeoch, D.J., et al., "The Complete DNA Sequence of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1," J. Gen. Virol. 69:1531-1574 (1988).
Ray, R., et al., "Mithramycin Blocks Protein Binding and Function of the SV40 Early Promoter," J. Clin. Invest. 83:2003-2007 (1989).
Snyder, R.C., et al., "Mithramycin Blocks Transcriptional Initiation of the c-myc P1 and P2 Promoters," Biochemistry 30:4290-4297 (1991).
Van Dyke, M.W., and Dervan, P.B., "Chromomycin, Mithramycin, and Olivomycin Binding Sites on Heterogeneous Deoxyribonucleic Acid--Footprinting with (Methidiumproply-EDTA)ironIII)," Biochemistry 22:2273-2377 (1983).
Youngquist et al. (1985) J. Am. Chem. Soc. 107:5528-9.
Gursky et al. (1983) Cold Spring Harbor Symp. Quant. Biol. 47:367-78 (vol. date 1982).
Birg et al. (1990) Nucleic Acids Research 18:2901-8.
Laugaa et al. (1985) Biochemistry 24:5567-75.
Kuhlman and Moser (1981) J. Med. Chem. 24:1333-7.
Montenay-Garestier (1991) CIBA Foundation Symposium 158:147-57.

Affiliated with

Also associated with

Rating

Sequence-directed DNA-binding molecules compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sequence-directed DNA-binding molecules compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequence-directed DNA-binding molecules compositions and methods will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1529790