Seminal plasma ribonuclease and method for its recovery

Details Seminal plasma ribonuclease and method for its recovery Seminal plasma ribonuclease and method for its recovery

1980-03-26

1982-05-25

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 6, 435172, C12N 922

Patent

active

043317642

ABSTRACT:
The new enzyme, seminal plasma ribonuclease, has a content of 1 mole of cysteine per mole, a K.sub.M of 0.42 mM against poly-r-U, a K.sub.M of 0.51 mM against poly-r(A-U) transcript, and is inactive against native RNA. It is obtained either by placing dialyzed seminal plasma on an anion exchanger and then on a cation exchanger and purifying the fraction not adsorbed on the cation exchanger by affinity chromatography, after a gel chromatography step if desired, or by subjecting it directly to affinity chromatography, a support material being used for the affinity chromatography which bears native RNA or a non-hydrolyzable analog of the enzyme substrate.

REFERENCES:
Methods in Enzymology, vol. 6, pp. 828-829, (1963).

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