Process for production of ascorbate oxidase

Details Process for production of ascorbate oxidase Process for production of ascorbate oxidase

1980-12-16

1982-05-25

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C12N 904

Patent

active

043317634

ABSTRACT:
Ascorbate oxidase is produced by extraction from plants of the genus Sechium, particularly the species thereof which is Sechium edule Sw. The crushed plant tissues are extracted with an aqueous alkaline solvent, preferably at about pH 11. The extract is then subjected to centrifugation, concentration under vacuum, salting out with ammonium sulfate and solvent fractionation with acetone, to prepare crude ascorbate oxidase, which is then further purified by dialysis, ion exchange chromatography, adsorption chromatography and gel filtration. The ascorbate oxidase thus obtained has an optimum pH of about 7, a km value of 0.3 mM, an isoelectric point of around pH 6.3 and a molecular weight of about 100,000.

REFERENCES:
patent: 4160696 (1979-07-01), Wu
"Enzyme Handbook", vol. 1, p. 229, by Thomas E. Barman; published 1969.
"Enzyme Handbook", pp. 158-159, Editor Shiro Akabari, Publisher K. K. Asakura Publ., 1st ed. Apr. 30, 1966-2nd Dec. 20, 1966.
Makino's New illustrated Flora of Japan", by Tomitaro Makino, Dr. Sc., 1961, pp. 11 and 612.

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