Selective inactivation of Aspergillus proteases

Details Selective inactivation of Aspergillus proteases Selective inactivation of Aspergillus proteases

1998-05-12

2000-06-27

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Enzyme inactivation by chemical treatment

435183, 435814, C12N 999, C12N 900

Patent

active

060805641

ABSTRACT:
A method is presented for inactivating undesired acid labile proteases which are expressed into the culture medium simultaneously with desired enzymes by Aspergillus species when Aspergillus species is used as the host for recombinantly producing enzymes. The method involves incubating culture medium which contains a mixture of the desired recombinantly produced enzyme and the undesired acid labile protease which have been expressed therein at pH values below 4.5 and at temperatures from 2.degree. C. to 75.degree. C. for at least 20 seconds.

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