Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-07-31
1998-02-17
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C12Q 1168, C12P 2100, C12N 996, C07K 14435
Patent
active
057190216
ABSTRACT:
A method is disclosed for producing a biochemically active polypeptide from a biochemically inactive polypeptide. The polypeptide is normally but need not be expressed in a precursor form containing a pro-sequence. The inactive polypeptide is reacted with a tailor-made activating peptide. The activating peptide can be synthetic or made by recombinant DNA procedure. The activating peptide is a peptide which contains one or more functional domains which are necessary for folding the inactive polypeptide into a biochemically active conformation. The activating peptide may but need not contain a sequence of amino acids which is identical to the sequence of the natural occurring pro-sequence of the polypeptide. Also, a method is disclosed which permits to identify the one or more functional domains in the pro-sequence of a polypeptide which contribute(s) to the folding of the inactive polypeptide into a biochemically active conformation. The invention relates also to a tailor-made activating peptide (synthetic or by recombinant DNA) and to the biochemically active polypeptide. The protein activation method and the biochemically active proteins are of major utility in their broad applications.
REFERENCES:
patent: 5191063 (1993-03-01), Inouye et al.
Silen et al. (1989) Nature, vol. 341, pp. 462-464.
Suter et al. (1991) EMBO Journal, vol. 10, No. 9, pp. 2395-2400.
Zhu et al. (1989) Nature, vol. 339, pp. 483-484.
Degen Nancy J.
Fleisher Mindy
University of Medicine and Dentistry of New Jersey
LandOfFree
Protein activation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Protein activation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protein activation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1783275