Saponin-digesting enzymes, genes thereof and soyasapogenol B...

Details Saponin-digesting enzymes, genes thereof and soyasapogenol B... Saponin-digesting enzymes, genes thereof and soyasapogenol B...

2006-04-04

2006-04-04

Pruuty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S254110, C435S320100, C435S325000, C435S252300, C435S254600, C435S052000, C435S254300, C536S023200

Reexamination Certificate

active

07022508

ABSTRACT:
The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

REFERENCES:
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S. Kudou et al., “Purification and some properties of soybean saponin hydrolase fromAspergillus oryzaeKO-2”, Agric. Biol. Chem., Jan. 1991, vol. 55, No. 1, pp. 31-36.
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Accession No. 1979: 143769, E.D. Biosis Perepelista et al., “Use of hydrolytic enzymes of the fungusaspergillus nigerBKMT-33 to increase diosgenin yield fromtribulus terrestris”, Prikl Biokhim Mikrobiol, 1978, vol. 14, No. 2, pp 309-312.

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