Salmonella enterica strains of reduced pathogenicity,...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C424S258100, C424S282100

Reexamination Certificate

active

08071356

ABSTRACT:
The invention concerns novelSalmonella entericaattenuated strains, characterized in that they are inactivated at the level of the znuABC operon through mutation in at least one of the znuA, znuB, znuC genes of such operon, for uses in medical or veterinary fields as vaccines.

REFERENCES:
patent: 6248329 (2001-06-01), Chandrashekar et al.
Campoy et al 2002 vol. 70 No. 8 pp. 4721-4725.
Ellis (Chapter 29 of Vaccines, Plotkin, et al. (eds) WB Saunders, Philadelphia, 1998, especially p. 571, paragraph 2).
2nd Annual ASM conference Salmonella: From Pathogenesis to therapeutics Sep. 2006, B17 Ammendola et al, Role of Zinc in the Host-Salmonella interaction pp. 1-105.
Dec. 27, 2007, International Search Report in PCT/IT2007/000410.
Written Opinion in PCT/IT2007/000410
Aug. 1, 2002, Campoy, Susana et al: “Role o f the high-affinity zinc uptake znuABC system inSalmonella entericaserovar typhimurium virulence,”Infection and Immunity, vol. 70, No. 8, Aug. 2002, pp. 4721-4725; XP002453141; ISSN: 0019-9567.
Apr. 1, 2003, Garrido Melena et al: “The high-affinity zinc-uptake system znuACB is under control of the iron-uptake regulator (fur) gene in the animal pathogenPasteurella multocida,” FEMS Microbiology Letters, vol. 221, No. 1, Apr. 1, 2003 (Apr. 11, 2003), pp. 31-37; XP002453142; ISSN: 0378-1097.
Jun. 1, 1998, Patzer Silkeiet al: “The ZnuABC high-affinity zinc uptake system and its regulator ZurinEscheri chiacoli ,” Molecular Microbiology, vol. 28, No. 6, Jun. 1998, pp. 1199-1210; XP002453143; ISSN: 0950-382X.
Oct. 1, 1999, Lewis DA et al: “Identificiation of the znuA-encoded periplasmic zinc transport protein ofHaemophilus ducreyi,” Infection and Immunity, American Society for Microbiology, Washington, US, vol. 67, No. 10, Oct. 1999, pp. 5060-5068; XP002253820; ISSN: 0019-9567.
Sep. 1, 2004, Kim Suk et al: “Zinc uptake system (znuA locus) of Bruce1 1 a abortus is essential for intracellular survival and virulence in mice,”Journal of Veterinary Medical Science, vol. 66, No. 9, Sep. 2004, pp. 1059-1063, XP002453144 ISSN: 0916-7250.
Jul. 1, 2006, Yang Xinghong et al: “Deletion of znuA virulence factor attenuatesBrucella abortusand confers protection against wild-type challenge,”Infection and ImmunityJul. 2006, vol. 74, No. 7, pp. 3874-3879, XP002453145; ISSN: 0019-9567.

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