Retroviral vectors pseudotyped with SRV-3 envelope glycoprotein

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...

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4351723, 435 696, 435236, 424 932, 4242071, C12N 700, C12N 1500, C12P 2104, A01N 6300

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059324678

ABSTRACT:
Cells producing recombinant retroviral particles are provided. The cells contain a first vector having a coding region encoding retroviral LTRs and a packaging signal under the control of an expression control system, a tRNA binding site located upstream from the packaging signal and origin of second strand DNA synthesis located downstream from the packaging signal. The cells also contain a second vector having a coding region encoding retroviral capsid proteins gag and pol under the control of an expression control system and a third vector having a coding region encoding a simian type D retrovirus envelope glycoprotein under the control of an expression control system.

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