Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-11-30
1991-01-01
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 435 694, 435 697, 435 701, 4351721, 4351723, 935 9, 935 10, 935 13, 935 76, C12Q 168, C12P 2100, C12N 1500
Patent
active
049817841
ABSTRACT:
A novel retinoic acid receptor is disclosed. The novel receptor is encoded for by cDNA carried on plasmid phRAR1, which has been deposited with the American Type Culture Collection for patent purposes. Chimeric receptor proteins are also disclosed. The chimera are constructed by exchanging functional domains between the glucocorticoid, the mineralocorticoid, the estrogen-related, the thyroid and the retinoic acid receptors. In addition, a novel method for identifying functional ligands for receptor proteins is disclosed. The method, which takes advantage of the modular structure of the hormone receptors and the idea that the functional domains may be interchangeable, replaces the DNA-binding domain of a putative novel receptor with the DNA-binding domain of a known receptor such as the glucocorticoid receptor. The resulting chimeric construction, when expressed in cells, produces a hybrid receptor whose activation of a ligand-(e.g., glucocorticoid) inducible promoter is dependent on the presence of the new ligand. The novel method is illustrated in part by showing that the ligand for the new receptor protein is the retinoid, retinoic acid.
REFERENCES:
Grun et al (1987) Nature 325:75-78.
Evans Ronald M.
Giguere Vincent
Ong Estelita
Segui Prudimar S.
Thompson Catherine C.
Brown Anne
Schwartz Richard A.
The Salk Institute for Biological Studies
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