Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1996-03-01
2000-01-18
Wortman, Donna C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, 536 2372, 536 2433, C12Q 170, C12Q 168
Patent
active
060156635
ABSTRACT:
A test based on restriction enzyme analysis identifies and differentiates strains of porcine reproductive and respiratory syndrome virus (PRRSV). Amplified cDNA from the ORF 5 region of the viral genome has been targeted for identification of unique restriction sites that allow for the differentiation of the vaccine strain from field strains, and for differentiation of field strains from each other through the use of selected restriction enzymes. This assay is useful for both clinical diagnosis of PRRSV field strains in vaccinated pigs as well as for epidemiological studies in the evaluation of the source and transmission of PRRS field viruses.
REFERENCES:
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Andreyev Vladimir G.
Clouser Deborah F.
Lager Kelly M.
Mengeling William L.
Vorwald Ann C.
Fado John D.
Ribando Curtis P.
Silverstein M. Howard
The United States of America as represented by the Secretary of
Wortman Donna C.
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