Recombinant vector capable of increasing secretion of Koji...

Details Recombinant vector capable of increasing secretion of Koji... Recombinant vector capable of increasing secretion of Koji...

2006-09-19

2010-11-30

Rao, Manjunath N (Department: 1656)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S069100, C435S254110, C435S254300, C435S320100, C530S358000

Reexamination Certificate

active

07842799

ABSTRACT:
To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like.Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.

REFERENCES:
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Machida, M., et al., Dec. 2005, “Genome sequencing and analysis ofAspergillus oryzae”, Nature, vol. 438, pp. 1157-1161.
Database Embl 1998, XP002503912, Database accession No. AF080600.
Database Embl 2001, XP002503913, Database accession No. AF284062.
Database Embl 2001, XP002503914, Database accession No. AAF11955.
Akimitsu Tanaka, “KoujikinAspefillus oryzaeYurai Alkaline Protease Idenshi no Hatsugen Kiko no Kaiseki” The Society for Biotechnology, Japan Taikai Koen Yoshishu, Aug. 24, 2001, p. 370, No. 953.
S. Cheevadhanarak, Cloning and selective overexpression of an alkaline protease-encoding gene fromAspergillus oryzae, Gene (1991), vol. 108, pp. 151-155.
Database EMBL, Jun. 18, 2004, “EST836955Aspergillus flavusNormalized cDNA Expression LibraryAspergillus flavuscDNA clone NAFEN88 5′ end, mRNA sequence” XP002568754 retrieved from EBI accession No. EMBL:C0142284.
Database EMBL, Mar. 13, 1997, “Emericella nidulansDNA binding regulatory protein AmdX (amdX) gene, complete cds.”, XP002568751 retrieved from EBI accession No. EMBL:U56100.
Database Geneseq, Mar. 28, 2003, “Aspergillus oryzaepolynucleotide SEQ ID No. 2520.”, XP002568753 retrieved from EBI accession No. GSN:ABZ53407.
Database Geneseq. Nov. 4, 2004, XP002568752 retrieved from EBI accession No. GSN:ADR84958.
European Search Report issued on Mar. 4, 2010 for European Application No. 09010747.5.

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