Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-01-11
2005-01-11
Swartz, Rodney P (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069300, C424S093400, C424S184100, C424S190100
Reexamination Certificate
active
06841358
ABSTRACT:
A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin ofBordetella(Fha) fused within the reading frame to a sequence (2) located upstream from the first sequence. Sequence (2) codes for at least part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein, which contains the interaction site of Fha and heparin and the secretion domain. This Fha protein is under the control of a promoter recognized by the cell polymerases ofB. pertussisand is inserted into aB. pertussiscell culture, is expressed in the culture and excreted into the cell culture medium. The invention uses both the abilities ofBordetellaand particularlyB. pertussisto secrete or surface expose the heterologous polypeptide fused to the Fha portion corresponding to sequence (2), which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from otherBordetellaproteins.
REFERENCES:
patent: 6036960 (2000-03-01), Relman et al.
patent: 0 453 216 (1991-10-01), None
patent: 453216 (1991-10-01), None
patent: 9004641 (1990-05-01), None
Menozzi et al. FEMS Microbiology Letters 78:59-64, 1991.*
Locht et al. Molecular Microbiology 9(4):653-660, 1993.*
Menozzi et al. Infection and Immunity 62(3): 769-778, 1994.*
Delisse-Gathoye et al. Infection and Immunity 58(9):2895-2905, 1990.*
Guzman et al. Microbial Pathogenesis 12:383-389, 1992.*
Miller, Jeff F., et al,Journal of Bacteriology, vol. 171, No. 11, 1989, pp. 6345-6348 “Analysis ofBordetella pertussisVirulence Gene Regulation by Use of Transcriptional Fusions inEscherichia coli”.
Miller, Jeff F., et al,Journal of Bacteriology, vol. 174, No. 3, 1992, pp. 970-979, “Constitutive Sensory Transduction Mutations in theBordetella pertussis bvgSGene”.
Roy, Craig R., et al,Journal of Bacteriology, vol. 173, No.7, 1991, pp. 2385-2392, “Identification ofBordetella pertussisRegulatory Sequences Required for Transcriptional Activation of the fhaB Gene and Autoregulation of the bvgAS Operon”.
Delisse-Gathoye, Anne-Marie, et al,Infection and Immunity, vol. 58, No. 9, 1990, pp. 2895-2905, “Cloning, Partial Sequence, Expression, and Antigenic Analysis of the Filamentous Hemagglutinin Gene of Bordetella pertussis”.
Menozzi, Franco D., et al,Infection and Immunity, vol. 62, No. 3, Mar. 1994, pp. 769-778, “Heparin-Inhibitable Lectin Activity of the Filamentous Hemagglutinin Adhesin ofBordetella pertussis”.
Willems, Rob J.L., et al,Molecular Microbiology, vol. 11, No. 2, Jan. 1994, pp. 337-347, “Mutational analysis of theBordetella pertussisfim/fha gene cluster: identification of a gene with sequence similarities to haemolysin accessory genes involved in export of FHA”.
Stibitz, Scott, et al,Journal of Bacteriology, vol. 170, No. 7, 1988, pp. 2904-2913, “Genetic Analysis of a Region of theBordetella pertussisChromosome Encoding Filamentous Hemagglutinin and the Pleiotropic Regulatory Locus vir”.
Domenighini, M., et al,Molecular Microbiology, vol. 4, No. 5, 1990, pp. 787-800, “Genetic characterization ofBordetella pertussisfilamentous haemagglutinin: a protein processed from an unusually large precursor”.
Locht, Camille, et al,Molecular Microbiology, vol. 9, No. 4, 1993, pp. 653-660, “The filamentous haemagglutinin, a multifaceted adhesin produced by virulent Bordetella spp.”.
Capron Andre
Jacob-Dubuisson Francoise
Locht Camille
Menozzi Franco
Renauld Genevieve
Institut Pasteur De Lille
Nixon & Vanderhye P.C.
Shahnan-Shah Khatol
Swartz Rodney P
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