Recombinant preparation of calcitonin fragments and use thereof

Details Recombinant preparation of calcitonin fragments and use thereof Recombinant preparation of calcitonin fragments and use thereof

1996-02-06

1999-10-05

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 681, 4352523, 4353201, 536 231, 530300, C12P 2104, C12P 2106, C12P 2100

Patent

active

059622709

ABSTRACT:
A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.

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