Recombinant enzyme for fragmenting N-acetylheparosan

Details Recombinant enzyme for fragmenting N-acetylheparosan Recombinant enzyme for fragmenting N-acetylheparosan

1995-06-07

1998-10-13

Grimes, Eric

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435201, 435 84, A61K 3847, C12N 926, C12P 1926

Patent

active

058208577

ABSTRACT:
The invention relates to a DNA fragment of sequence S.sub.1 as described in the application and encoding the S.sub.2 peptide sequence as described in the application. This DNA sequence carries the gene encoding the enzyme for fragmenting N-acetylheparosan and the adjacent sequences permitting its expression. The invention also relates to an enzyme intended for fragmenting N-acetylheparosan derived from this gene and the processes for fragmenting high molecular mass N-acetylheparosan using this enzyme.

REFERENCES:
patent: 5482844 (1996-01-01), Salome et al.
patent: 5550116 (1996-08-01), Lormeau et al.
patent: 5583027 (1996-12-01), Salome et al.
Dhirenda S. Gupta et al., "Enzymatic degradation of the capsular K5-antigen of E. coli by coliphage K5", FEMS Microbiology Letters, vol. 16, No. 1, Jan. 1983, p. 13-17.

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