Recombinant DNA compounds and expression vectors encoding para-n

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 691, 435 712, 435 43, 4352523, 43525233, 4353201, 4351723, 536 232, 935 14, 935 29, 935 43, 935 56, 935 73, C12N 1555, C12N 918, C12N 121, C12N 1563

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054686326

ABSTRACT:
The present invention provides isolated DNA compounds and recombinant DNA cloning and expression vectors that encode PNB esterase from Bacillus subtilis. The invention also provides host cells transformed with these vectors and a method for production of the PNB esterase by recombinant DNA techniques.

REFERENCES:
patent: 3972774 (1976-08-01), Brannon
patent: 4757012 (1988-07-01), Estell et al.
patent: 4839293 (1989-06-01), Cantor et al.
Berger, S. L. et al. (eds.) "Guide to Molecular Cloning Techniques" Meth. in Enzymology vol. 152 pp. 393-399, 415-423, 432-447 661-704 (1987).
Belyansky, A. et al. "PCR based cDNA library construction: . . . " Nuc. Acids Res. vol. 17(8):2919-2932 (Apr. 1989).
Deutscher, M. P. (ed.) "Guide to Protein Purification". Meths. in Enzymol. vol. 182 pp. 9-15, 285-421, 459-477, 602-613, 738-751 (1990).
J. Sambrook et al. (eds.) "Molecular Cloning: A Laboratory Manual" Cold Spring Harbor Laboratory Press (Oct. 1989) Chapter 17, pp. 17.1-17.44.
Brannon, D. R., et al., J. Antiobiotics, vol. 29(2), pp. 121-124 (1976).

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