Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-01-11
2000-08-08
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435176, 435243, 435261, G01N 3353, C12N 1114, C12N 100, C12N 102
Patent
active
061000434
ABSTRACT:
Cloning systems useful for the isolation of recombinant nucleic acid are disclosed in which the recombination of cloning-system nucleic acid and foreign nucleic acid is linked to the expression of a moiety on the surface of a host organism, the moiety being a first member of a binding pair. When recombination occurs between the nucleic acid and the foreign nucleic acid, the moiety is expressed on the surface of the host organism. The isolation of recombinant nucleic acid is then performed by attaching a second member of the binding pair to a solid support and contacting the host organism with the support. When the first member of the binding pair is expressed on the surface of the host organism, the host organism binds to the second member of the binding pair attached to the solid support, thereby selectively isolating those organisms.
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Brandis John
Bridgham John A.
Hoeprich, Jr. Paul D.
Leong John
O'Neill Roger A.
Bortner Scott R.
Degen Nancy
Grossman Paul D.
Sandals William
The Perkin-Elmer Corporation
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