RecA nucleoprotein filament and methods

Details RecA nucleoprotein filament and methods RecA nucleoprotein filament and methods

1987-01-20

1989-12-19

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435803, 435810, 4351723, 935 78, 935 28, 536 27, 436808, C12Q 168, C07H 2100, C12N 1500

Patent

active

048882747

ABSTRACT:
A stable, single-stranded nucleoprotein filament adapted to complex specifically and stably with a target duplex DNA having a selected base sequence. The filament is composed of a single-stranded DNA probe having a region of homology with the target base sequence, and RecA protein bound stably to the DNA probe by adenosine 5'-(.gamma.-thio)triphosphate. The filament is useful in a novel system and method for enriching target duplex DNA which contains a region homologous to the probe sequence, for blocking selected restriction endonuclease sites in the target DNA, and in other DNA methodologies in which stable rapid triple-strand synaptic formation in duplex DNA can be exploited.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4395486 (1983-07-01), Wilson et al.
Bryant, F. R. et al, Proc. Natl. Acad. Sci. USA, vol. 82, 1985, pp. 297-301.
Flory, J. et al, Proc. Natl. Acad. Sci. USA, vol. 81, 1984, pp. 7026-7030.
Riddles, P. W. et al, J. Biol. Chem., vol. 260, 1985, pp. 170-173.

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