Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-07-24
1994-07-26
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436536, 436537, 436544, 436546, 436815, G01N 33542, G01N 33533
Patent
active
053326610
ABSTRACT:
Immunoassay methods and reagents for the quantification of total doxepins (i.e., E-doxepin, Z-doxepin, E-desmethyldoxepin, and Z-desmethyldoxepin) in a test sample are disclosed. The quantification of total doxepins is accomplished in an immunoassay employing antibodies and labeled reagents prepared with doxepin derivatives of the Formula II: ##STR1## wherein Y--Z can be C.dbd.CH or N--CH.sub.2, R.sub.` is a linking group, R.sub.2 can be H or CH.sub.3, and Q can be a detectable moiety or an immunogenic carrier material. The antibody reagent comprises antibodies which are capable of binding to total doxepins and which are produced with one or more immunogens prepared from the doxepin derivative of Formula II, and the labeled reagent is also prepared from the doxepin derivative of Formula II.
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Adamczyk Maciej
Fishpaugh Jeffrey R.
Hruska Robert E.
Johnson Donald
Abbott Laboratories
Ceperley Mary E.
Collins Daniel W.
Steele Gregory W.
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