Reagent compositions for use in the identification and character

Details Reagent compositions for use in the identification and character Reagent compositions for use in the identification and character

1992-10-15

1995-08-01

Czaja, Donald E.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 8, 436 10, 436166, 436172, 435 2, G01N 3348

Patent

active

054380030

ABSTRACT:
A reagent composition which includes organic cationic dyes for staining the reticulocytes in a blood sample and buffer solutions for maintaining a pH of about 6 to about 9 is provided. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. A zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes. The reagent composition and whole blood sample mixture are passed through the sensing region of a flow cytometer. The light scattered and absorbed by each cell is measured; the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

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