Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1983-09-16
1986-07-29
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 435 24, 435 28, 435805, 435810, C12Q 100, C12Q 128, C12Q 136, C12Q 148
Patent
active
046031073
ABSTRACT:
The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity.
The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents:
REFERENCES:
patent: 2905594 (1959-09-01), Morris
patent: 4177109 (1979-12-01), Tohyama et al.
patent: 4425427 (1984-01-01), Luderer
patent: 4511651 (1985-04-01), Beaty et al.
Bergmeyer, Methoden der Enzymatischen Analyse, vol. I, (1974), Verlag Chemie, Weinheim, pp. 757-759 and 761.
Deneke Ulfert
Nagel Rolf
Rittersdorf Walter
Boehringer Mannheim GmbH
Warden Robert J.
White Patricia K.
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