Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-06-24
1994-02-15
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 701, 4352401, 530350, C12N 506, C12P 2102
Patent
active
052866459
ABSTRACT:
The present invention relates to a novel cell line isolated from a rat osteosarcoma having the following characteristics: A) a non-expressed p53 protein; B) a normal RB-1 gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; C) a normal c-fos gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; D) a 50-fold amplified c-myc gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; E) a monolayer MATRIGEL.TM. growth pattern; F) tumorigenic in nude mice; G) normal alkaline phosphatase activity; H) an ability to be serially propagated greater than fifty population doublings; I) an ability to produce a growth factor selected from the group consisting of: 1) a mesenchymal inhibitory growth factor; 2) a non-heparin binding mitogenic growth factor; 3) a first heparin binding mitogenic growth factor; 4) a second heparin binding mitogenic growth factor; 5) a third heparin binding mitogenic growth factor; and 6) a fourth heparin binding mitogenic growth factor.
The present invention also relates to growth factors produced by the novel cell line.
This invention also relates to a process for producing such growth factors.
REFERENCES:
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Doersen Claus-Jens W.
Isfort Robert J.
Corstanje Brahm J.
Graff IV Milton B.
Hill Jr. Robert J.
Suter David L.
Teng Sally
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