Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-06-24
1994-02-15
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 701, 4352401, 530350, C12N 506, C12P 2102
Patent
active
052866432
ABSTRACT:
The present invention relates to a novel cell line isolated from a rat osteosarcoma wherein the cell line has the following characteristics: a) a normal p53 gene; b) a normal RB-1 gene; c) a normal c-myc gene; d) a normal c-fos gene; e) a deregulated immediate early gene response; f) a canalicular network MATRIGEL.TM. growth pattern; g) poorly tumorigenic in congenitally athymic mice; h) no alkaline phosphatase activity; i) an ability to produce one or more of the following growth factors: 1) a non-heparin binding growth factor, 2) a first heparin binding growth factor, 3) a second heparin binding growth factor, and 4) a third heparin binding growth factor; and j) an ability to be serially propagated greater than sixty population doublings.
The present invention also relates to growth factors having the characteristics of growth factors isolated from the rat osteosarcoma cell line.
The present invention further relates to a process for producing such growth factors comprising growing the cells of the present invention in a culture medium and recovering the growth factors.
REFERENCES:
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Doersen Claus-Jens W.
Isfort Robert J.
Corstanje Brahm J.
Graff IV Milton B.
Hill Jr. Robert J.
Suter David L.
Teng Sally P.
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