Rapid mutation testing system for human cells

Details Rapid mutation testing system for human cells Rapid mutation testing system for human cells

1985-07-08

1988-06-28

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 435 32, 435 34, 4351721, 4353171, 536 27, 935 24, 935 33, 935 79, C12N 100, C12N 1500, C12Q 102

Patent

active

047538740

ABSTRACT:
Mutagenic capability is determined by employing a shuttle vector having prokaryotic and eukaryotic origins, a prokaryotic marker an a gene capable of screening or selection in a prokaryote. The method involves introducing the vector into mammalian cells, exposing the cells to the candidate to be tested for mutagenicity for a time sufficient to allow lesions to occur, rescuing the vector by transforming into a prokaryotic host and screening for mutations of the gene.

REFERENCES:
patent: 4486533 (1984-12-01), Lambowitz
Seidman, M. M., et al., "A Shuttle Vector Plasmid . . . ", Gene 38, 233-237 (1985).
Calos, M. P., et al., "High Mutation Frequency in DNA . . . ", Proc. Natl. Acad. Sci USA 80, 3015-3019 (May 1983).
Razzaque, A., et al., "Rearrangement and Mutagenesis . . ., ", Proc. Natl. Acad. Sci., USA 80 3010-3014 (May 1983).
Lebkowski, J. S. et al., "Transfected DNA is Mutated . . . Human Cells", Mol. Cell. Biol. 4(10), 1951-1960 (Oct. 1984).
Sarkar, S., et al., "Error-Prone Mutagenesis Detected in Mammalian Cells by a Shuttle Vector . . . ", Mol. Cell. Biol. 4(10), 2227-2230 (Oct. 1984).

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