Method for assaying proteases with tagged proteinaceous inhibito

Details Method for assaying proteases with tagged proteinaceous inhibito Method for assaying proteases with tagged proteinaceous inhibito

1985-07-26

1988-06-28

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 13, 435 23, 435184, G01N 33573, C12Q 156, C12Q 138, C12N 999

Patent

active

047538759

ABSTRACT:
A new and useful method for assaying proteases, in which an excess of tagged proteinaceous inhibitor of a protease is mixed in solution with a sample containing the protease to form a mixture of a tagged inhibitor-protease complex and free tagged inhibitor. The complex is separated from the tagged inhibitor and the tag measured. Alternatively, anti-protease antibody may be added to bind the complex so that assay specificity and/or separation is enhanced. The method of this invention is applicable to assaying minute quantities of clinically significant proteases e.g. renin, kallikrein, thrombin occurring in serum, plasma, urine and other tissues.

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