Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-10
2006-10-10
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07118867
ABSTRACT:
The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/μl.
REFERENCES:
patent: 5976842 (1999-11-01), Wurst
patent: 0 942 917 (1999-09-01), None
patent: 01 098 993 (2001-05-01), None
patent: WO 200005409 (2000-02-01), None
Dostal et al. (“An Improved Method for Absolute Quantification of mRNA Using Multiplex Polymerase Chain Reaction: Determination of Renin and Angiotensinogen mRNA Levels in Various Tissues” Analytical Biochemistry. 1994. vol. 223: pp. 239-250).
Kellogg et al (“TaqStart Antibody: Hot Start PCR Facilitated by a Neutralizing Monoclonal Antibody Against Taq DNA Polymerase” BioTechniques. 1994. vol. 16, No. 6: pp. 11-34-1137).
Lin et al. (“Inhibition of Multiple Thermostable DNA Polymerases by a Heterodimeric Aptamer” Journal of Molecular Biology. 1997. 271: pp. 100-111).
Vet et al. (“Multiplex detection of four pathogenic retroviruses using molecular beacons” Proc. Natl. Acad. Sci. May 1999. vol. 96: pp. 6394-6399).
Wong et al. (“Monitoring mRNA Expression by Polymerase Chain Reaction: The “Primer-Dropping” Method” Analytical Biochemistry. 1994. 223: pp. 251-258).
Henegariu et al. (“Multiplex PCR: Critical Parameters and Step-by-Step Protocol” BioTechniques. Sep. 1997. 23: pp. 504-511).
Hahn, S., et al., 2000, “Multiplex and Real-Time Quantitative PCR on Fetal DNA in Maternal Plasma, A comparison with Fetal Cells Isolated from Maternal Blood,”Annals New York Academy of Sciences, 906:148-152.
“Highly Efficient and Specific RT-PCR with a new combination of QIAGEN enzymes”,QIAGEN NEWS, Online, 1999, 4:15-16.
“FastStart Taq DNA Polymerase: end your chase for the most specific hot start PCR results”,Roche Diagnostics GmbH, Roche Molecular Biochemicals Online, Aug. 2000.
Ryncarz, A., et al., 1999, “Development of a High-Throughput Quantitative Assay for Detecting Herpes Simplex Virus DNA in Clinical Samples”,Journal of Clinical Microbiology, 37(6):1941-1947.
Vet, J., et al., 1999, Multiplex detection of four pathogenic retroviruses using molecular beacons,Proc Natl Acad Sci, USA, 96:6394-6399.
Betzl Gisela
Desilva Deepika Marine
Millward Hallegh Page
Rasmussen Randy P.
Soong Richie
Babic Christopher M
Doyle Charles M.
Horlick Kenneth R.
Idaho Technology, Inc.
Roche Diagnostics Corporation
LandOfFree
Quantitative multiplex PCR with high dynamic range does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Quantitative multiplex PCR with high dynamic range, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Quantitative multiplex PCR with high dynamic range will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3719752