Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1999-03-26
2000-05-23
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
530350, 435 74, 435 75, G01N 3353, G01N 33573, C07K 113, C12Q 148
Patent
active
060664622
ABSTRACT:
A method of quantitating the activity of a selected protein kinase on a peptide substrate is provided. The peptide substrate is conjugated to a binding compound. The modified peptide substrate is then added to a solution containing the selected protein kinase. The protein kinase and the peptide are incubated along with a label for sufficient time to form a modified peptide product having the binding compound and the label. The modified peptide product is then bound to a matrix having a high binding compound affinity. The bound peptide is then washed and the activity of the protein kinase is measured. Also provided is a kit for the stated method.
REFERENCES:
patent: 4923802 (1990-05-01), Gallis
patent: 5235039 (1993-08-01), Heath, Jr. et al.
Kemp. Design and Use of Peptide Substrates for Protein Kinases. Methods in Enzymology. 200: 121-134, 1991.
Guzo David
Promega Corporation
Shuman Jon D.
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