Purification process for hirudin using affinity chromatography

Details Purification process for hirudin using affinity chromatography Purification process for hirudin using affinity chromatography

1995-09-06

1997-12-16

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 514 21, 530324, 530344, 530413, 530416, 530417, 530855, B01D 1508

Patent

active

056981040

ABSTRACT:
Disclosed herein is a process for purifying hirudin from a solution containing hirudin and other substances, characterized in that the process comprises a step of subjecting the solution to a metal ion affinity chromatography wherein copper ion (Cu.sup.++) is used as a metal ion and a phosphate buffer is used as an eluent.

REFERENCES:
patent: 4551271 (1985-11-01), Hochuli
patent: 4832849 (1989-05-01), Cardin
patent: 5034133 (1991-07-01), Naveh
patent: 5077388 (1991-12-01), Tang
patent: 5095092 (1992-03-01), Badziong
patent: 5169936 (1992-12-01), Staples
patent: 5179196 (1993-01-01), Johnson
patent: 5304310 (1994-04-01), Lang
patent: 5372719 (1994-12-01), Afeyan
Markwardt, "Hirudin as an Inhibitor of Thrombin", Meth. Enzymol., vol. 19:924-932, (1970).
Porath et al., "Metal Chelate Affinity Chromatography, A New Approach to Protein Fractionation", Nature, vol. 258:598-599,(1975).
Bagdy et al., "Hirudin", Meth. Enzymol., vol. 45:669-678, (1976).
Markwardt et al., "Pharmacokinetics and Anticoagulant Effect of Hirudin in Man", Thromb Haemostas (Stuttgart), vol. 52(2): 160-163, (1984).
Walsmann et al., "On the Isolation of the Thrombin Inhibitor Hirudin", Thrombosis Research, vol. 40:563-569, (1985).
Mao et al., "Rapid Purification and Revised Amino-Terminal Sequence of Hirudin: A Specific Thrombin Inhibitor of the Bloodsucking Leech", Analytical Biochemistry, vol. 161:514-518, (1987).
Riehl-Bellon et al., "Purification and Biochemical Characterization of Recombinant Hirudin Produced by Saccharomyces Cerevisiae", Biochemistry, vol. 28:2941-2949, (1989).
Fareed et al., "An Objective Perspective on Recombinant Hirudin: A New Anticoagulant and Antihrombotic Agent", Blood Coagulation and Fibrinolysis, vo. 2:135-147, (1991).
Jung-Hoon et al., "Gene Expression and Secretion of the Anticoagulant Hirudin in Saccharomycess Cerevisiae," Journal of Microbiology and Biotechnolgy, vol. 1(4):266-273, (1991).
Misawa et al., "High Level Expression and Secretion of Biologically Active Leech Hirudin Variant 1 (HVI) by Escherichia Coli,"Proceeding of ApBioch. EC., pp. 62-64, (1992).

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