Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1991-11-20
1994-05-03
Brown, Johnnie R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
435 71, 435 74, 435 696, 435 692, 435810, 435962, 435975, 436 69, 436 86, 530413, C12Q 156, G01N 3386
Patent
active
053087569
ABSTRACT:
A chromogenic assay for determination of Protein S, using an indicator of Factor Xa as a measure of Protein S concentration. This technique utilizes the dependency of activated Protein C on Protein S in order to inactivate a known portion of Factor VIII. Residual Factor VIII is then activated and acts with Factor IXa to activate Factor X. The chromogenic substrate is then cleaved by Factor Xa and the rate of conversion of the indicator molecule is an indirect indicator of free Protein S.
REFERENCES:
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patent: 5001069 (1991-03-01), Bartl et al.
patent: 5147805 (1992-09-01), Preda et al.
Thrombosis Research 63(1) 189-193 (1991).
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High, K. A.; "Antithrombin III, Protein C, and Protein S" Arch. Pathol. Lab. Medicine; 112 (Jan.), 28-36 (1988).
Kobayashi, I. et al.; "Functional Activity of Protein S determined with Use of Protein C Activated by Venom Activator"; Clinical Chemistry; 35 8, 1644-1648 (1989).
Koedam, J. A. et al., "Inactivation of human factor VIII by Activated Protein C" J. Clinical Investigation; 82 4, 1236-1243 (1988).
Preda, L. et al.; "A Prothrombin Time-Based Functional Assay of Protein S"; Thrombosis Research; 60, 19-32 (1990).
Rick, M. E. et al.; "Factor IXa and von Willebrand factor modify the inactivation of factor VIII by activated protein C"; J. Lab Clin. Med. 115 4, 415-421, (1990).
Suzuki, K and Nishioda, J.; "Plasma Protein S Activity Measured Using Protac, a Snake Venom Derived activator of Protein C"; Thrombosis Research; 49 2, 241=251, (1988).
van der Waart, P. et al; "A Functional Test for Protein S Activity in Plasma" Thrombosis Research; 48, 427-437 (1987).
van de Waart Piet
Woodhams Barry J.
Baxter Diagnostics Inc.
Brown Johnnie R.
Bucklo Michael P.
Pearson Louise S.
Tymeson Cynthia G.
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