Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-06-02
1997-07-22
Wax, Robert A.
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 4352523, 43525231, 536 231, 536 241, 530324, 935 10, 935 27, 935 29, 935 41, 935 48, C12N 1500, C12N 1563, C12N 1574, C12N 1575
Patent
active
056503261
ABSTRACT:
The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.
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Wells, J. A., et al., in The Genetics and Biotechnology of Bacilli, vol. 2, "Cloning and sequencing of a region controlling efficient expression of subtilisin from Bacillus amyloliquefaciens", pp. 173-180. 1983
Wong, S.-L., et al., Proceedings of the National Academy of Sciences, U.S.A., vol. 81, "The subtilisin E gene of Bacillus subtilis is transcribed from a sigma37 promoter in vivo", pp. 1184-1188. 1984.
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Aaslyng Dorrit Anita
Dambmann Claus
Outtrup Helle
Sloma Alan P.
Agris, Esq. Cheryl H.
Moore William W.
Novo Nordisk A S
Novo Nordisk Biotech Inc.
Wax Robert A.
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