Prolonged culturing of avian primordial germ cells (PGCs) using

Details Prolonged culturing of avian primordial germ cells (PGCs) using Prolonged culturing of avian primordial germ cells (PGCs) using

1997-08-04

2000-12-05

Campell, Bruce R

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Avian cell, per se

435377, 435325, 800 21, 800 8, C12N 1585, C12N 500, A01K 6700

Patent

active

061565695

ABSTRACT:
A culture system for maintaining avian PGCs for long periods in tissue culture is provided. This culture system uses LIF, bFGF, IGF and SCF. The resultant PGCs are useful for the production of transgenic and chimeric avians, in particular, chickens or turkeys.

REFERENCES:
patent: 5759763 (1998-06-01), Naito et al.
Pain et al. Long-term in vitro Culture and Characterisation of Avian Embryonic Stem Cells with Multiple Morphogenic Potentialities. Development, vol. 122, pp. 2339-2348, Aug. 1996.
Sang, H. Transgenic Chickens-Methods and Potential Application. TIBTECH, vol. 12, pp. 415-420, Oct. 1994.
Maclean, N. (Ed.), Animals with Novel Genes. Simkiss, K. Transgenic birds. University Press. pp. 106-137, 1994.
Alberts et al. (Ed.). Molecular Biology of The Cell. Garland Press. p. 162, 1989.

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