Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1995-06-07
1996-05-21
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
435 691, 4353201, 435 711, 435 712, 435183, C12P 1306, C12N 1501, C12N 1570, C12N 900
Patent
active
055189067
ABSTRACT:
A method of producing D-pantothenic acid or a salt thereof characterized by bring a microbe belonging to the family Enterobacteriaceae having resistance to salicylic acid and capable of producing D-pantothenic acid in the presence of .beta.-alanine in contact with .beta.-alanine, preferably wherein a microbe resistant to .alpha.-ketoisovaleric acid and/or .alpha.-ketobutyric acid, and/or .alpha.-aminobutyric acid and/or .beta.-hydroxy-aspartic acid and/or O-methyl-threonine or a microbe transformed with a plasmid DNA carrying the region of a gene involved in biosynthesis of pantothenic acid or a salt thereof or a part thereof, is used, and a method of producing D-pantoic acid or a salt thereof characterized by culturing a microbe resistant to salicylic acid, .alpha.-ketoisovaleric acid and/or .alpha.-ketobutyric acid and/or .alpha.-aminobutyric acid and/or .beta.-hydroxy-aspartic acid and/or O-methyl-threonine and capable of producing D-pantoic acid to accumulate D-pantoic acid or a salt thereof, which is then harvested, and in accordance with these methods, D-pantothenic acid, D-pantoic acid or salts thereof can be efficiently directly obtained microbiologically without using DL-pantoic acid, DL-pantolactone etc. as starting materials.
REFERENCES:
Maas, "Pantothenate Studies II. Evidence from Mutants for Interferance by Salicylate with Pantoate Synthesis", J. Bacter. 63: 227-232 (1952).
Vallari et al "Preparation of High-Specific-Activity D-[3-.sup.3 H] Pantothenic Acid" Analyt. Biochem. 154:671-675 (1986).
Maas et al, "Pantothenate Studies I. Interference by D-Serine and L-Asparte Acid with Pantothenate Synthesis in E. coli", J. Bacter. 60:733-745 (1950).
Foulds et al "Decreased Permeation of Cephalosporins Through The Outer Membrane of E. coli Grown in Salicylates", Antimicrob. Agnts. Chemo. 33(4):412-417 (Apr. 1981).
Adelberg et al, "Optimal Conditions for Mutagenesis by N-methyl-N'-nitroso-guanidine in E. coli K12", Biochem.Biophs.Res.Comm 18:788-795 (1965).
Kawabata et al, "Enzymatic Synthesis of Pantothenic Acid by Escherichia coli Cells", ACS Symp. Ser. 106 (Immobilized Microb. Cells):133-137 (1979).
Kawabata et al "Enzymatic Synthesis of Pantothenic Acid by Escherichia coli cells", Enzyme Engin. 5:389-391 (1980).
Teller, Biosynthesis of Pantoate, as abstracted in Diss. Abstr. Int. B 31(10): 5804, (1971).
Cronan et al "Genetic and Biochemical Analyses of Pantothenate Biosynthesis in Escherichia coli in", J. Bacter. 149(3):916-922 (Mar. 1982).
Pantothenate Studies--Description Of The Extracted Pantothenate-Synthesizing Enzyme Of Escherichia Coli By Werner K. Maas, J. Biol. Chem. 198, 23 (1952) pp. 23-32.
Extracellular Production Of Pantothenic Acid By Escherichia Coli Cells--Abstract Papers, Am. Chem. Sec. 176 Meet. Micr. 48/1978, Kawabata et al., abstract #48.
Hikichi Yuichi
Miki Hiroshi
Moriya Takeo
Nogami Ikuo
Yamaguchi Takamasa
Carlson K. Cochrane
Takeda Chemical Industries Ltd.
Wax Robert A.
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