Production method and preparation method of glucans

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S194000, C435S195000, C536S123120

Reexamination Certificate

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10475943

ABSTRACT:
A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.

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Takata et al.; “Purification and Characterization of α-Glucan Phosphorylase fromBacillus Stearothermophilus”; Journal of Fermentation and Bioengineering; vol. 85, No. 2; pp. 156-161; 1998.
Takaha; “Structure and Properties ofThermus Aquaticusα-Glucan Phosphorylase Expressed inEscherichia Coli”; J. Appl. Clycosci; vol. 48, No. 1; pp. 71-78; 2001.
Waldman, et al.; “The Enzymic Utilization of Sucrose in the Synthesis of Amylose and Derivatives of Amylose, Using Phosphorylases”; Carbohydrate Research, 157 (1986).

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