Producing pullulanase with organisms having a superior capacity

Details Producing pullulanase with organisms having a superior capacity Producing pullulanase with organisms having a superior capacity

1974-02-25

1976-06-15

Monacell, A. Louis

Textiles: fiber preparation

195 62, 195 65, 195 66R, 195112, 195114, C12D 1302, C12D 1310, C07G 702

Patent

active

039635759

ABSTRACT:
Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

REFERENCES:
patent: 3654088 (1972-04-01), Coker et al.
patent: 3766014 (1973-10-01), Masuda et al.
patent: 3806419 (1974-04-01), Heady
patent: 3827940 (1974-08-01), Sugimoto et al.

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