Processes for preparing acarviosyl transferase and for using it

Details Processes for preparing acarviosyl transferase and for using it Processes for preparing acarviosyl transferase and for using it

1997-03-14

1999-11-23

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4352523, 43525411, 4353201, 435325, 435410, 536 232, C12N 910, C12N 1500, C12N 120, C07H 2104

Patent

active

059898828

ABSTRACT:
The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.

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