Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1988-02-08
1989-11-14
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, C12Q 102
Patent
active
048807320
ABSTRACT:
The invention relates to a process and an equipment for the rapid determination of the spermium count of sperm samples and/or the ratio of the living spermia. The process comprises dissolving the fluorescent dyestuff propidium iodide in a buffer and measuring the intensity (F.sub.o), then adding the sperm sample and, after measurement of the intensity (F.sub.1) and addition of a cytoplasm membrane-permeabilizing agent, determining the intensity (F.sub.2), then adding a buffer and a permeabilizing agnet to the buffer and measuring the intensity (F.sub.3) and subsequently the intensity (F.sub.4) of the pure buffer and finally the intensity (F.sub.5) of the sperm mixture added to the buffer, and calculating the cell concentration by using the formula: ##EQU1## wherein .alpha. is the multiplication product from the slope of the calibration curve with the predilution ratio; and calculating the living cell ratio by using the formula: ##EQU2##
REFERENCES:
patent: 4559309 (1985-12-01), Evenson et al.
patent: 4683213 (1987-07-01), Ax
Blazak et al.-Chem. Abst., vol. 97 (1982), p. 106458y.
Damjanovich Sandor
Gaspar Rezso
Matko Janos
Resli Istvan
Szollosi Janos
INNOFINANCE Altalanos Innovacios Penzintezet
Rosen Sam
LandOfFree
Process for the rapid determination of sperm cell count and/or l does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for the rapid determination of sperm cell count and/or l, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the rapid determination of sperm cell count and/or l will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1852679