Process for the preparation of incoagulable blood by means of pr

Details Process for the preparation of incoagulable blood by means of pr Process for the preparation of incoagulable blood by means of pr

1981-04-20

1983-05-31

Yoncoskie, Robert A.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

426 32, 260112B, C07G 700

Patent

active

043861610

ABSTRACT:
Proteolytic enzymes, particularly papain, are used for rendering whole blood incoagulable. The treatment is preferably carried out at a pH of 7.2 with a ratio between enzyme and substrate, for instance, 1:200, and a volume ratio of 1:10. In the case of papain, a 1.6% solution is advantageously used. The incoagulable blood may be used to prepare a protein concentrate by precipitating the proteins with an agent, for instance, acetone or ethanol, preferably at 0.degree. C. in the presence of hydrochloric acid.

REFERENCES:
patent: 1403892 (1922-01-01), Butterfield
patent: 2456297 (1948-12-01), Melnick
patent: 2471282 (1949-05-01), Paddock
patent: 3046197 (1962-07-01), Harper
patent: 3672954 (1972-06-01), Grippa
patent: 3928630 (1975-12-01), Perini
patent: 4262022 (1981-04-01), Christensen et al.

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