Process for the preparation of human monoclonal antibodies and t

Details Process for the preparation of human monoclonal antibodies and t Process for the preparation of human monoclonal antibodies and t

1996-04-03

1998-08-25

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 691, 435325, 4351723, 4353201, 530350, C12P 2104, C12P 2106

Patent

active

057982309

ABSTRACT:
The present invention provides a process for the preparation of human monoclonal antibodies from human B cells. According to this process, human antibody-producing B cells are immortalized by infection with an Epstein-Barr virus, or derivative thereof, that expresses Epstein-Barr virus nuclear antigen 2 (EBNA2). EBNA2 function, which is required for immortalization, is subsequently shut off, which results in an increase in antibody production. The invention provides a variety of systems that allow expression of EBNA2 function to achieve immortalization, followed by reduction of EBNA2 expression or function to facilitate antibody production.

REFERENCES:
Goossens, D. et al. 1987. J. Immunol. Methods, 101:193-200.
Cohen, J. I et al. 1989. Proc. Natl. Acad. Sci. USA, 86:9558-9562.
Benjamin, D. et al. 1982. J. Immunol.129:1336-1342.
Picard, D. et al. 1988. Cell; 54:1073-1080.
Kempkes, B. et al., "B-cell proliferation and induction of early G.sub.1 -regulating proteins by Epstein-Barr virus mutants conditional for EBNA2", The EMBO Journal, vol. 14, No. 1, 88-96 (1995).

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