Process for the isolation of alkaloid components from the plant

Details Process for the isolation of alkaloid components from the plant Process for the isolation of alkaloid components from the plant

1978-05-22

1979-10-23

Moyer, Donald B.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

546 51, C07D51904, C07D21500, C07G 500

Patent

active

041720776

ABSTRACT:
Tumor inhibiting alkaloids are recovered from Vinca Rosea L. by a method which comprises extracting the dried leaves with a solvent selected from the group consisting of an alkanol having 1 to 5 carbon atoms, a mixture of an alkanol having 1 to 5 carbon atoms and a dilute, aqueous solution, benzene and benzene homologs; subsequently purifying the alkaloid extract obtained by a phase-change method between immiscible solvents, precipitating the main amount of dimeric alkaloids in form of their sulphuric acid addition salts, isolating leurosine, vincistine, vinblastine, desacetoxy-vinblastine, N-desmethyl-vinblastine and desacetyl-vinblastine from the salt mixture obtained, and then completing the separation and isolation of the remaining alkaloids by separation and isolation by adjusting the pH-value of the mother liquor, after precipitation and removal of the salt mixture, to 5.5 to 10, extracting the solution with a water-immiscible organic solvent and separating vindoline, catharantine, 3',4' -anhydrovinblastine and leurosine from the extract by chromatography, extracting vindoline in the pH-range of 2.5 to 3.5 and catharantine 3',4'-anhydrovinblastine and leurosine in the pH-range of 5 to 6 with benzene or a benzene homologue, from the mother liquor obtained when isolating the salt mixture, and separating a mixture of leurosine and 3',4'-anhydrovinblastine by crystallization, epoxidizing this mixture with an oxygen source, or separating the mixture into its components by chromatography, and subsequently epoxidizing the 3',4'-anhydrovinblastine component to leurosine, and isolating catharantine remaining from the mother liquor obtained when separating the crystalline mixture, or separating the extract containing catharantine, 3',4'-anhydrovinblastine and leurosine into its components by chromatography.

REFERENCES:
patent: 3097137 (1963-07-01), Beer et al.
patent: 3205220 (1965-09-01), Svoboda et al.
patent: 3225030 (1965-12-01), Svoboda
patent: 3352868 (1967-11-01), Neuss et al.
patent: 3370057 (1968-02-01), Svoboda et al.
patent: 3392173 (1968-07-01), Hargrove
patent: 3422112 (1969-01-01), Gorman et al.
Svoboda et al., J. of Pharm. Sci., Aug. 1962, vol. 51, No. 8, pp. 707-720.

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