Process for the biotechnical production of rhamnolipids includin

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435100, 435105, 435249, 435874, 536 182, 536 41, C07G 1306, C12P 1944, C12P 1912, C12P 1902

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048142727

ABSTRACT:
Rhamnolipids with high surface activity are produced micro iologically in high yield per g of dry cell substance using Pseudomonas spec. DSM 2874 in the form of growing, resting and immobilized cell mass in an aqueous medium containing at least one assimilable carbon source at a pH of 6.7 to 7.3 and a temperature of 30.degree. to 37.degree. C. Two new rhamnolipids with only one .beta.-hydroxydecanoic acid residue in the molecule and defined as .alpha.-L-rhamnopyranosyl- and 2-O-.alpha.-L-rhamnopyranosyl-.alpha.-L-rhamnopyranosyl-.beta.-hydroxydeca noic acid with a molecular weight of 334 and 480, respectively, are obtained.

REFERENCES:
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Syldatk et al., "Production of Four Interfacial Active Rhamnolipids from n-alkanes or Glycerol by Resting Cells of Pseudomonas Species, DSM 2874", Z. Naturforsch, 40c:61-67 (1985).
Wagner et al., "Production of Surface Active Anionic Glycolipids by Resting and Immobilized Microbial Cells", Eur Congr. Biotechnol., 3rd, 1984 1:3-8 (Abstract).
Mandelstam et al., Part II, Chapter 2, "Growth: Cells and Populations", Biochemistry of Bacterial Growth, Blackwell Scientific Publications, Boston, 1982, pp. 120-122.
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