Process for tempering tissue for heparin production

Details Process for tempering tissue for heparin production Process for tempering tissue for heparin production

1978-04-05

1980-02-12

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435 84, 536 21, C12B 100, C08B 3710

Patent

active

041884671

ABSTRACT:
An improved method of tempering frozen heparin-bearing animal tissue is disclosed wherein the frozen tissue is particulated, thawed and warmed in a heat exchanger and fermented at temperatures ranging from about 60.degree.-85.degree. F. for 5-12 hours. Putrefaction and odor are avoided; the tissue has improved uniformity in biochemical content and is capable of high heparin yields when processed according to known procedures. When the tempered tissue is defatted with an azeotropic solvent, more of the fat is removed and the defatted tissue is more permeable to heparin recovery solutions and heparin isolation is facilitated.

REFERENCES:
patent: 2410084 (1946-10-01), Kuizenga
patent: 2884358 (1959-04-01), Bush et al.
patent: 3862003 (1975-01-01), Okuyama et al.

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