Process for sulphation and phosphorylation of proteins and pepti

Details Process for sulphation and phosphorylation of proteins and pepti Process for sulphation and phosphorylation of proteins and pepti

1986-09-05

1987-11-10

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

530303, 530304, 530345, 530404, 530406, 530408, 530410, A61K 3726, C07K 740, C07K 1500

Patent

active

047058455

ABSTRACT:
A process for sulphation and phosphorylation of a protein or peptide comprising contacting said protein or peptide with sulphuric or phosphoric acid in the presence of a non-aqeuous apolar organic solvent and contacting the resultant solution with a dehydrating agent is disclosed. Non-aggregating insulin products with bioactivity may be prepared by this process.

REFERENCES:
patent: 3361574 (1968-01-01), Paulsen
patent: 4322344 (1982-03-01), Chen et al.
patent: 4534894 (1985-08-01), Cerami et al.
Biochim-Biophys. ACTA 5, 89-97 (1950), Fraenkel-Contrat et al.

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