Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1989-09-12
1992-11-10
Moezie, F. T.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530351, 530416, 530417, 530418, 530419, C07K 300, C07K 312
Patent
active
051625075
ABSTRACT:
A process for recovering highly pure, recombinant IL-2 from transformed microorganisms in which the cells are disrupted; impure recombinant IL-2 is isolated in the form of refractile bodies from the disruptate; the impure IL-2 is dissolved and denatured with at least 6M guanidine hydrochloride containing a reducing agent; the reduced IL-2 is precipitated and resolubilized; the reduced solubilized IL-2 therein is oxidized by a controlled oxidation; the oxidized IL-2 is refolded by reducing the concentration of guanidine hydrochloride in the solution; and the oxidized, refolded IL-2 is further purified by ion exchange chromatography or hydrophobic interaction chromatography and ion exchange chromatography.
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Davis John T.
Dorin Glenn J.
Lim Amy
Smith Flint
Weissburg Robert
Cetus Corporation
McGarrigle Philip L.
Moezie F. T.
Murphy Lisabeth F.
Rozycki Andrew G.
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