Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid
Patent
1983-07-22
1987-09-22
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving uric acid
435 11, 435 14, 435 25, 435 28, 435810, C12Q 162, C12Q 160, C12Q 154, C12Q 126, C12Q 128
Patent
active
046955399
ABSTRACT:
A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
REFERENCES:
patent: 4353983 (1982-10-01), Siddiqi
Stryer L. (1975), Biochemistry, 2d ed., p. 344.
Porras et al. (1981), The Reaction of Reduced Xanthine Oxidase with Oxygen, J. Biol. Chem., 256(17): 9096-9103.
Matkovics et al. (1975), Utilization of Catalase and Superoxide Dismutase to Clarify the Mechanism of Action of Oxidases, Proc. Hung Annu. Meet. Biochem., 15: 95, Chem. Abstr. 88: 46907w.
Galliana et al. (1980), Formation of Superoxide Radical.
Galliani et al. (1980), J. Chem. Soc. Perkins Trans II, 1:1-3.
Halliwell et al. (1982), Interaction of the Superoxide Radical with Peroxidase and with Other Iron Complexes, Oxidase Relat. Redox System Proc. Int. Symp., 3rd, 1979.
Hamanaka Tadashi
Hanada Toshiro
Kodera Hiroyuki
Miki Yutaka
Miyashita Yoshibonu
Kepplinger Esther M.
Wako Pure Chemical Industries Ltd.
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