Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases
Patent
1989-10-17
1994-08-02
Wityshyn, Michael G.
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Hydrolases
424 941, 424 9464, 435212, 435216, 435814, 435815, 435885, 514822, C12N 102, A61K 37547
Patent
active
053343840
ABSTRACT:
A process for the separation of streptokinase from contaminating proteins in a streptokinase-containing mixture, which comprises treating the mixture with a reducing agent to reduce disulphide bridges in the contaminating proteins to free thiol groups, contacting the mixture with a reagent R-X wherein R is a group capable of reacting with a free thiol group and X is a group R.sup.1 capable of reacting with a free thiol group or is a thiol-containing matrix, and thereafter separating the resulting chemically modified contaminating proteins from the mixture to provide streptokinase in a form substantially free of contaminating proteins.
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Harber Julian S.
Lewis Ceri J.
Mannix Christopher J.
Smith Richard A. G.
Beecham Group p.l.c.
Williams Jane
Wityshyn Michael G.
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