Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2005-11-08
2005-11-08
Navarro, Mark (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
active
06962803
ABSTRACT:
The present invention provides a method of purifying diphtheria toxin comprising (1) fermenting a microorganism strain capable of producing diphtheria toxin using glucose as a carbon source, said method comprising adding glucose to a growing culture whereby the addition of glucose maintains microorganism growth effective to support diphtheria toxin production; and (2) purifying the diphtheria toxin from the culture by contacting a toxin containing preparation derived therefrom with an ion exchange matrix, eluting a fraction containing the toxin, applying the eluate to a hydrophobic matrix, and eluting a fraction containing the toxin.
REFERENCES:
patent: 4711845 (1987-12-01), Gelfand et al.
patent: 4925792 (1990-05-01), Rappuoli
patent: 5728383 (1998-03-01), Johnson et al.
Rappuoli, et al. “Rapid Purification of nDiphtheria Toxin by Phenyl Sepharose and DEAE Cellulose Chromatography” Journal of Chromatography, vol. 268, 1983 pp. 543-548 XP002140797.
Webm et,ak, :Diphtheria Toxin-Related Alpha-Melanocyte-Stimulating Hormone Fusion Toxin The Journal of Biological Chemistry, vol. 266, No. 19, Jul. 5, 1991, pp. 12289-12293 XP002140798.
Black Christopher
Hellebust Halldis
Merchant Fahar
Merchant Rosemina
Stokke Geir
Amersham Health AS
Cai Li
Navarro Mark
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