Process for producing polypeptide having disulfide bond

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S069800, C435S069700, C435S069100, C435S232000, C435S320100, C435S252300, C435S252310, C536S023200, C536S023500, C536S023400, C530S303000

Reexamination Certificate

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07037684

ABSTRACT:
The invention relates to a DNA that enables co-expression of a protein disulfide isomerase and a polypeptide having disulfide bonds. The invention further relates to a process of producing polypeptides using the DNA and a process of enhancing the efficiency of formation of correct disulfide bond in an expression system of a gene recombinant polypeptide having disulfide bonds.

REFERENCES:
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patent: 00 66738 (2000-11-01), None
Winter et al. , J. Biotechnol. 84:175-185, 2000.
A. Miyauchi et al.: “Pilot scale production of a recombinant human epidermal growth factor, secreted byBacillus brevis, using expanded bed adsorption” J. Indust. Microbiol. Biotech., vol. 21, pp. 208-214 1998.
Tsutomu Kajino et al.: “A protein disulfide isomerase gene fusion expression productivity ofBacillus brevis” Applied and Environmental Microbiology, vol. 66, No. 2, pp. 638-642 Feb. 2000.
Hideaki Tojo et al.: “Production of human protein disulfide isomerase byBacillus brevis” Journal of Biotechnology, vol. 33, No. 1, pp. 55-62 1994.
S. Udaka et al.: “High-level secretion of heterologous proteins byBacillus brevis” Methods in Enzymology, vol. 217, pp. 23-33 1993.

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