Process for producing peptides in E. coli

Details Process for producing peptides in E. coli Process for producing peptides in E. coli

1994-12-05

1997-09-23

Grimes, Eric

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 681, 435 691, 435 697, C12P 2100, C12P 2102, C12P 2106

Patent

active

056703406

ABSTRACT:
The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises:

REFERENCES:
patent: 4987070 (1991-01-01), Magota et al.
"Bacterial Synthesis of Recombinant .alpha.-Human Atrial Natriuretic Polypeptides", Yoshimasa Saito et al., Journal of Biochemistry, pp. 111-122, vol. 102, No. 1, Jul. 1987, Tokyo, Japan.
"Chemical Synthesis and Cloning of a Poly(arginine)-Coding Gene Fragment Designed to Aid Polypeptide Purification", C. Smith et al., Gene, pp. 321-327, vol. 32, 1984. Amsterdam NL.
Tajima, M. et al.; J. Fermantation Bioeng. 72:362-367 (Nov. 1991).
Sung. W.L. et al.; Proc. Natl. Acad. Sci. USA 83:561-565 (1986).
Guo et al, "Synthesis of human insulin gene VIII. Construction of expression vectors for fused proinsulin production in Escherichia coli" Gene, 29 pp. 251-254 (1984).
Schein, "Production of Soluble Recombinant Proteins in Bacteria", Biotechnology, vol. 7, pp. 1141-1147 (1989).
Skoog et al, "Calculation of the isoelectric points of polypeptides from the amino acid composition", Trends Anal. Chem., vol. 5, No. 4, pp. 82-83 (1986).
Itakura et al, "Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin", Science, vol. 198, pp. 1056-1063 (1977).
Goeddel et al, "Expression of Escherichia coli of chemically synthesized genes for human insulin", Proc. Natl. Acad. Sci., USA, vol. 76, No. 1, pp. 106-110 (1979).
Shine et al, "Expression of cloned .beta.-endorphin gene sequences by Escherichia coli, Nature," vol. 285, pp. 456-461 (1980).

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