Process for producing cis-3-hydroxy-L-proline

Details Process for producing cis-3-hydroxy-L-proline Process for producing cis-3-hydroxy-L-proline

2006-09-26

2006-09-26

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S189000, C435S320100, C435S832000, C435S886000, C536S023200

Reexamination Certificate

active

07112432

ABSTRACT:
An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

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