Process for nucleic acid hybridization and amplification

Details Process for nucleic acid hybridization and amplification Process for nucleic acid hybridization and amplification

1990-05-07

1993-06-29

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 935 77, 935 78, C12P 1934

Patent

active

052234142

ABSTRACT:
The present invention is directed to a method of achieving RecA protein facilitated amplification of a double-stranded DNA target sequence having first and second complementary strands, each strand with 5' and 3' ends. The method involves complexing a primer complementary to a 5' end region of the first strand and a primer complementary to a 5' end region of the second strand with RecA protein in the presence of ATP-.gamma.-S. The complexed primers are then reacted in a mixture also containing the target sequence, all four dNTPs, RecA protein and DNA polymerase. The reaction is conducted below the temperature required for thermal dissociation of the two target strands and continued until a desired degree of amplification of the target sequence is achieved.
The present invention further includes the cloning and identification of the coding sequences for the RecA protein of Thermus aquaticus.

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